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Andy Lee takes 2nd Prize for his Poster at UK Surface Probe Microscopy (SPM) 2013

This event, organised by the Royal Microscopical Society (RMS) (www.rms.org.uk), covered a wide range of topics associated with scanning probe microscopy (SPM). The meeting supported discussions covering ultra-high resolution & high speed SPM, materials analysis & surface property mapping, biological AFM and novel SPM techniques. A large variety of eminent speakers represented their respective fields, with plenary speakers included; Prof. Toshio Ando, Prof. Daniel Mueller, Prof. Klaus Kern, Dr Bart Hoogenboom, Prof. Michael Edwardson and many others.

Andy Lee presented a poster entitled; “A Single Molecule Interrogation Approach to the RecA Mediated Patterning of DNA Nanoarchitectures”, taking 2nd prize in the poster competition for his work. Andy said, “The conference was a fantastic opportunity to meet some my hero’s in the field and to be able to discuss my work with them.” Commenting on the poster prize, “It was completely unexpected, there was some fantastic work being presented. But certainly a welcome surprise.”

Poster abstract:

RecA mediated patterning of DNA scaffolds is proposed as a self-assembling route to bottom up nano-scale device construction, surpassing the limitations of traditional top-down approaches. In order to take this system from the established 1D patterning system to address 2D & 3D DNA nano-architectures, it is necessary to understand how RecA is able to mediate a homology search. Here, preliminary AFM work to assess the time course and influences of experimental parameters is presented. It is found that despite confinement in 2D upon a charged surface, the nucleoprotein filament maintains its ability to form sequence specific triple stranded DNA complexes. Furthermore, the interaction occurs in a highly concurrent manner with nucleoprotein filament invasions occurring rapidly while the resolution of multiplexed invasions is taking place at a substantially longer time-scale.